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Previous study showed that peracetic acid fog was an effective disinfection technology for the inactivation of common bacteria. In this study, the effects of peracetic acid fog on the stability and integrity of purified human epidermal keratinocyte (NHEK) cells were investigated. NHEK cells suspended in F12K culture medium were exposed to vapors of 2% peracetic acid/12% acetic acid, and the vapors were then passed through a biological safety cabinet (BSC) for removal of active oxidants. After BSC removal, viable cell counts and propidium iodide staining were used to assess cellular integrity and cell cycle progression. NHEK cells were injured by exposure to peracetic acid fog. However, cell viability was not significantly affected when the cells were exposed to 4°C or below for up to 60 min at 2% peracetic acid concentration. When the exposure time to peracetic acid vapor was up to 1 h at 2% concentration, cellular mortality was similar to bacterial disinfection with 81555fee3f

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